Evaluation of an identification method for the SARS-CoV-2 Delta variant based on the amplification-refractory mutation system

The Delta variant of SARS-CoV-2 dominated the COVID-19 pandemic due to its high viral replication capacity and immune evasion, causing massive outbreaks cases, hospitalizations, deaths. Currently, identification is performed mainly by sequencing. However, requirements for equipment operators as well cost have limited application in underdeveloped regions. To achieve an economical rapid method suitable undeveloped areas, we applied amplification-refractory mutation system (ARMS) based on PCR detection novel coronavirus variants. results showed that this could be finished 90 min detect few 500 copies/mL not react with SARS-Coronavirus, influenza A H1N1(2009) , other cross-pathogens or influenced fresh human blood, α- interferon, interfering substances. In a set double-blind trials, tests 262 samples obtained from patients confirmed infection revealed our was able accurately identify sensitivity specificity. conclusion, ARMS-PCR rapid, sensitive, specific, economical, areas. future study, will further variants, such Omicron.